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vacuum centrifugal concentrator  (Eppendorf AG)


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    Structured Review

    Eppendorf AG vacuum centrifugal concentrator
    Vacuum Centrifugal Concentrator, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 201118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vacuum+centrifugation/Eppendorf/pm42130394-87-18-23
    Average 99 stars, based on 201118 article reviews
    vacuum centrifugal concentrator - by Bioz Stars, 2026-10
    99/100 stars

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    other:

    Article Title: Multi-omic analyses reveal a differential contribution of chromatin-associated PP1 holoenzymes to mitotic exit and G1 re-establishment.
    Article Snippet: The protein samples were then reduced by the addition of 100 μl of 10 mM dithiothreitol (Sigma Aldrich, UK) in denaturation buffer for 30 min at ambient temperature, and alkylated by adding 100 μl of 55 mM iodoacetamide (Sigma Aldrich, UK) in denaturation buffer for 20 min at ambient temperature in the dark.

    Centrifugation:

    Article Title: Label–free quantitative urinary proteomics for non-invasive biomarker discovery in endometrial cancer
    Article Snippet: .. The resulting peptides were desalted using Pierce C18 spin columns (Thermo Scientific, Rockford, IL, USA) and dried via vacuum centrifugation (Eppendorf Concentrator plus TM, Hamburg, Germany). .. Finally, the peptide concentration was determined using a Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific, IL, USA) ( – ).

    Article Title: Specialisation of meiotic kinetochores revealed through a synthetic spindle assembly checkpoint strategy
    Article Snippet: .. Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). ..

    Article Title: Proteomic Profiling of Endometrial Cancer Tissues Reveals Differential Expression of Proteomes in Obese Versus Non-Obese Patients
    Article Snippet: The reaction was acidified with 7 μL of 10% formic acid and the samples were desalted using Pier-e C18 spin columns (Thermo Scientific, Mount Prospect, IL, USA). .. Following quantification with a Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific, Mount Prospect, IL, USA), the eluted peptides were dried using vacuum centrifugation (Eppendorf Concentrator plus TM, Hamburg, Germany) [20–22]. .. In the proteomics workflow, peptides were reconstituted in 0.1% (v/v) formic acid and processed through a Dionex UltiMate 3000 nano-LC system (Thermo Fisher Scientific, Germering, Germany) with a WPS-3000 autosampler (Thermo Fisher Scientific, Germering, Germany).

    Article Title: Dissection of the T cell infiltrate in mouse pancreatic tumors reveals an extensive and diverse tumor-reactive T cell repertoire
    Article Snippet: Briefly, cells that were pretreated for 2 days with IFN-γ were treated daily as follows for 5 consecutive days: The medium was removed from cells in a flask and then washed twice with 8 ml of Hanks’ balanced salt solution (+Mg, +Ca; Gibco, no. 14025092) to remove residual tissue culture medium. .. Peptides eluted in 28% acetonitrile (ACN) in 0.1% trifluoroacetic acid (TFA) were dried by vacuum centrifugation (Concentrator Plus, Eppendorf). .. The obtained peptides were desalted by reverse-phase purification using a SepPak 96-well plate (Waters, no. 186003966).

    Article Title: Establishment of a screening method for potential allergens and assessment of the allergenicity of Chlorella variabilis.
    Article Snippet: Chlorella variabilis NC64A (C. variabilis) is protein-rich microalgae with great application potential in food industry, but it also has certain risks, especially allergenic risks.. To ensure its safe application in food industry, an efficient screening method on potential allergens of C. variabilis was developed using bioinformatics tools like AllerBase, COMPARE, and AllerCatPro 2.0, and the allergenicity of C. variabilis was evaluated by serological, cytological, and animal experiments.. Results showed that 26 potential allergens were identified by combining proteomics with screening results, enabling efficient theoretical screening.

    Article Title: Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity
    Article Snippet: .. Eluted lysates in 40% acetonitrile/0.1% formic acid were dried by vacuum centrifugation (Concentrator Plus; Eppendorf) at 45°C. ..

    Article Title: Proteomic Profiling of Endometrial Cancer Tissues Reveals Differential Expression of Proteomes in Obese Versus Non-Obese Patients
    Article Snippet: The reaction was acidified with 7 μL of 10% formic acid and the samples were desalted using Pier-e C18 spin columns (Thermo Scientific, Mount Prospect, IL, USA). .. Following quantification with a Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific, Mount Prospect, IL, USA), the eluted peptides were dried using vacuum centrifugation (Eppendorf Concentrator plus TM, Hamburg, Germany) [ , , ]. .. In the proteomics workflow, peptides were reconstituted in 0.1% ( v / v ) formic acid and processed through a Dionex UltiMate 3000 nano-LC system (Thermo Fisher Scientific, Germering, Germany) with a WPS-3000 autosampler (Thermo Fisher Scientific, Germering, Germany).



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